Short answer: often, but not always. Freezing does not kill most yogurt cultures, it puts them to sleep. Whether a cup of frozen yogurt actually has live cultures depends on how the mix was made and handled. Here is what to look for, whether you are eating froyo or serving it.
Yogurt is milk fermented by two bacteria, Lactobacillus bulgaricus and Streptococcus thermophilus. Many makers add more cultures, such as L. acidophilus and Bifidobacterium. Frozen yogurt starts from a yogurt or yogurt-based mix, which is sweetened, chilled and churned in a soft-serve machine or freezer, the same way ice cream is.
The difference from ice cream is the cultured yogurt. That gives froyo its tang, and it is also where any live cultures come from.
Mostly no. Cold slows bacteria down rather than killing them. When yogurt freezes, the cultures go dormant. Some are lost to ice crystals and long storage, but a good share survive, and they become active again once they warm up after you eat them.
That is why the Live and Active Cultures seal for yogurt has a separate, lower standard for frozen yogurt: at least 10 million cultures per gram when it is made, compared with 100 million per gram for refrigerated yogurt.
The thing that kills cultures is heat, not cold. Some frozen yogurt mixes are pasteurized or heat-treated after the yogurt is cultured, to extend shelf life. That step wipes out most of the bacteria. Other "frozen yogurt" products contain very little yogurt at all.
Because the US has no federal standard for frozen yogurt, two cups that look the same can be very different. One can be full of live cultures and the other can have almost none.
Greek yogurt is strained regular yogurt, so it starts with the same cultures. Frozen Greek yogurt keeps them as long as it is not heat-treated after culturing. The same label check applies.
If you run a frozen yogurt shop, cafe or smoothie bar, the mix you choose decides whether you can honestly tell customers your froyo has live cultures.
Pumjil Original is a shelf-stable frozen yogurt mix powder made with real dehydrated Greek yogurt and 14 live and active cultures, 67 billion per bag. You mix it cold with milk and water, with no cooking step in your shop, and pour it straight into any soft-serve machine.
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It can be. Frozen yogurt made from a cultured mix that was not heat-treated afterward still carries live cultures. Check the label for "live and active cultures."
Usually fewer. Some cultures are lost in freezing and storage, which is why the Live and Active Cultures standard for frozen yogurt is lower than for refrigerated yogurt.
No. Freezing makes them dormant. Most survive, although the texture changes when it thaws.
Only if the maker added them. L. acidophilus is an extra culture, not one of the two that make yogurt, so look for it by name on the label.


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